Regulatory

Part:BBa_K2610004

Designed by: Charlotte de Ceuninck van Capelle, Marjolein Crooijmans   Group: iGEM18_Leiden   (2018-10-09)


pKatG

The KatG gene encodes for hydroperoxidase I. It protects aerobic, phosphate-starved cells from oxidative damage. The expression of KatG is activated by the global regulator OxyR. This enzyme is specifically upregulated by oxidative agents, as demonstrated by previous research. It has been shown that the promoter of KatG fused to the lux operon can be strongly induced by hydrogen peroxide in a dose-dependent response. (Zhang et al, 2015)(Mitchell et al, 2004)


Usage and Biology

iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1)


T--Leiden--heatmap.png
Figure 1. Heatmap with stress-activated promoters

As can be observed in Figure 1 the KatG promoter is very specifically activated by hydrogen peroxide. This specificity is integral to our screening system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 50
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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